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. 2018 Jun 29;13(6):e0199197. doi: 10.1371/journal.pone.0199197

Fig 4. Knock down of DCNL5 by siRNA has no effect on the activation of the NF-KB pathway.

Fig 4

(A) RAW264.7 were electroporated with siRNA and stimulated with LPS 24 hours later. Knockdown efficiency was measured by immunoblot. (B) As in (A) RAW264.7 were electroporated with siRNA and stimulated with Poly (I:C) 24 hours later for the indicated times. Total RNA was extracted and the mRNA encoding DCNL5, IFNβ, CXCL10, ISG15 and MX1 were measured by qRT-PCR as described under the “Experimental Procedures.” The results show relative mRNA levels compared with the value of 1.0 measured in unstimulated control cells. The experiment was performed in quadruplicate for each condition. Similar results were obtained in two independent experiments. Adjacent graphs show the means (± s.e.m) of quantified mRNA levels. Statistical significance was determined by two-ways ANOVA. P≤0.05 (C) RAW264.7 were electroporated with siRNA and stimulated with LPS 24 hours later. Knockdown efficiency was measured by immunoblot.