Table 2. Primers used for the molecular characterization of genetically modified parasites by PCR based analysis.
| Primer No. | Primer name | Primer sequence |
|---|---|---|
| 1. | Primer 1 | 5’TGTAGAAGTACTCGCCGATAGTGG 3’ |
| 2. | Primer 2 | 5’CATTTGCCACGCTCTGCCGTCAGTAGCA 3’ |
| 3. | Primer 3 | 5’CGCAGCTATTTACCCGCAGGACAT 3’ |
| 4. | Primer 4 | 5’AACGTGCTCATCTTTTCACTGTATAAGACGC 3’ |
| 5. | Primer 5 | 5’ATAGCGTTGGCTACCCGTGATATTGC 3’ |
| 6. | Primer 6 | 5’AACACGGCGGCATCAGAGCAGCCGATTG 3’ |
| 7. | Primer 7 |
5’GCGGACACACATAACCGGGAGTACTAG 3’ |
| 8. | Primer 8 |
5’TCCGCCGCCTTCTTCTTGCCAGACAT 3’ |
| 9. | ZeoFP | 5' ATGGCCAAGTTGACCAGTGCCGTTCC 3' |
| 10. | ZeoRP | 5' TCAGTCCTGCTCCTCGGCCACGAA 3' |