(A) Nfre rescues the nfre-1 nodulation phenotype. Nodules formed by Gifu wild type or nfre-1 plants at 7wpi with M. loti NZP2235 transformed with the empty vector or the wild type Nfre driven by the CaMV 35S promoter respectively. The number of plants analysed is shown (n). Error bars-standard error of the mean. **p<0.01, Student´s t-test. (B) Wild-type and nfre plants after 9 weeks growth on soil. Wild-type plants are larger, have more shoots (arrows), flowers and pods (arrowheads), while nfre plants are shorter, and have just started to develop flowers. (C) Shoot fresh weight (D) Total number of nodules formed when exposed to the native soil rhizobia. (E) The ratio of pink nodules formed when exposed to the native soil rhizobia. The number of plants analysed is shown at the bottom of each bar. Box plot graphs are shown with max and min values, and the significance was assessed by Kruskal-Wallis test. (F) The nodules formed by nfre mutants at 5 wpi have a wild-type appearance, and are fully colonized by M. loti- GFP.