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. 2018 Jun 18;128(7):3186–3197. doi: 10.1172/JCI98931

Figure 3. P4 administration does not recover implantation failure but rescues decidualization in Hif2a-uKO mice.

Figure 3

(A–C) Decidual prolactin-related factors Prl3c1 and Prlr, hallmarks of decidual health and luteotrophin, were reduced in Hif2a-uKO mice during decidualization. In A and C, n ≥ 4, *P < 0.05, mean ± SEM, Student’s t test. In B, scale bar, 200 μm; arrowhead, an embryo; arrow, a destroyed embryo with blood cell infiltration; dec, decidua. (D and E) Serum P4 levels were reduced in Hif2a-uKO mice compared with the controls on days 6 and 8 of pregnancy, whereas serum E2 levels were comparable between them. *P < 0.05, mean ± SEM, Student’s t test. (F and G) Daily injection of P4 from day 2 of pregnancy (2 mg/mouse/day) restored decidualization in Hif2a-uKO mice on day 8 of pregnancy. P > 0.05, mean ± SEM, Student’s t test. Arrow and arrowhead indicate implantation site and ovary, respectively. (H) P4 supplementation did not recover embryo survival in Hif2a-uKO mice on day 8 of pregnancy. No embryo was observed at the implantation site of Hif2a-uKO mice with P4 treatment. Scale bar, 200 μm; dec, decidua. (I–K) Hif2a-uKO mice with daily P4 injection showed the reduced weight of implantation sites on day 12, and did not deliver any pups in parturition. *P < 0.05, mean ± SEM, Student’s t test.