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. 2018 Jun 4;128(7):2951–2965. doi: 10.1172/JCI98727

Figure 5. SA2-mutated cancer cells, upon depletion of SA1, are defective in HR.

Figure 5

(A) Negative enrichment of the DNA repair gene set in the SA1-KD TC32 cells, determined by GO enrichment analysis. (B) Venn diagram showing overlaps of dysregulated DNA repair genes in the SA1-KD TC32 cells with the HRD gene signature. (C) Heatmap of clusters indicates that TC32 cells had increased levels of HRD upon SA1 KD, as analyzed by unsupervised clustering for HRD gene signature genes. (D) The SA2-mutated cancer cells are defective in the HR repair. Modified HR repair assay was performed by transfecting cells with DR-GFP DSB substrate and I-Sce I plasmids, and flow cytometry analysis was performed to detect GFP-positive cells. **P < 0.01; ***P < 0.001, Fisher’s exact test (B) and unpaired 2-tailed t test (D). Data are presented as mean ± SD and are representative of 3 independent experiments (D).