Figure 2. Human thymic precursors overexpressing active NOTCH1 undergo BM engraftment and extrathymic differentiation.

(A) Representative phenotype of primary ETPs from human postnatal thymus (n = 20). (B and C) RAG-2^–/– × γc^–/– mice were transplanted with human ETPs transduced with ICN1 and GFP (19.3% ± 1.4% ICN1⁺) or GFP alone (28.0% ± 7.9% GFP⁺). Shown are absolute numbers of human cells recovered from thymus (B) and BM (C) at the indicated weeks after transplant. Numbers were normalized to 10⁵ transduced input cells and represent combined results from 4 to 8 independent experiments, with a total of 35 to 75 mice per group. (D) Representative flow cytometry phenotype of human ICN1⁺ cells in BM and thymus of mice shown in B and C at 6 weeks after transplant (n = 4). (E) Representative CD44 expression of human DP TCR-αβ⁺ cells infiltrating the BM and thymus of mice shown in B and C (n ≥ 4). (F) Percentages of human CD45⁺ cells engrafting the BM of mice shown in B and C at the indicated times after transplant (n ≥4).