Figure 1. Hematopoietic cells are present in the brains of WT and BLT humanized mice.

(A) Flow cytometric analysis revealed the presence of murine hematopoietic cells (mCD45⁺) in the brains of BALB/c mice. Murine myeloid cells (mCD11b⁺), B cells (mCD19⁺), and T cells (mCD3ε⁺), including CD4⁺ and CD8⁺ T cell subsets, were present. (B) Representative flow cytometric plots from 2 of the BLT mice in Figure 2A demonstrating the presence of human hematopoietic cells (hCD45⁺), myeloid cells (hCD33⁺), B cells (hCD19⁺), and T cells (hCD3⁺), including CD4⁺ and CD8⁺ T cell subsets. (C) Phenotypic characterization of the human macrophages in the brains of BLT mice showed the presence of classical (CD14⁺CD16^–), intermediate (CD14⁺CD16⁺), and nonclassical (CD14^dimCD16⁺) macrophages (gating for hCD45⁺hCD11b⁺CD33⁺ cells).