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. 2018 Jun 4;128(7):2894–2913. doi: 10.1172/JCI96640

Figure 11. Inflammasome-related biomarkers are detected in human urine following contrast administration.

Figure 11

(A) Urinary IL-18 and (B) KIM-1 before and less than 24 hours after coronary angiogram as determined by Meso Scale Discovery assay. Data points normalized to urine creatinine are shown (IL-18 before vs. after, P = 0.005, n = 42/group; KIM-1 before vs. after, P = 0.03, n = 42/group, Wilcoxon’s signed-rank test). (C) Caspase-1 immunoblotting from urine pellets in 2 healthy control individuals and 8 patients before and less than 24 hours after coronary angiogram. Nigericin-treated THP-1 macrophages were used as a positive control. Arrow indicates cleaved capase-1 N-terminal CARD domain. (D) Caspase-1 immunoblotting from urine pellets in 9 patients before and less than 24 hours after coronary angiogram, with corresponding urine leukocyte score. (E) Quantification of caspase-1 expression versus leukocyte score in 17 patient urine samples before and less than 24 hours after coronary angiogram (caspase-1 densitometry normalized to urine creatinine per urine leukocyte score, 0 vs. 1: *P = 0.011, 0 vs. 2: ***P = 0.0001, n = 21, 7, and 6/group, ANOVA).