Figure 8. Characterization of inflammasome activation in infiltrating proinflammatory monocytes in CI-AKI.

LysM^(gfp/gfp) and Nlrp3^–/– LysM^(gfp/gfp) mice were treated with vehicle or ioversol and assessed at various time points. (A) LysM-GFP⁺CD11b⁺ leukocytes were isolated from vehicle- or ioversol-treated LysM^(gfp/gfp) mice at 6 hours and analyzed for inflammasome activation (active caspase-1) in infiltrating monocytes and resident macrophages by flow cytometry using CX₃CR1, Ly6C, F4/80, and CCR2. Images are representative of 3 independent experiments. (B) LysM-GFP⁺CD11b⁺CX₃CR1⁺Ly6C⁺ monocytes/macrophages were isolated from LysM^(gfp/gfp) and Nlrp3^–/– LysM^(gfp/gfp) mice treated with vehicle or ioversol and analyzed at 6 hours or 3 days using CCR2 and F4/80. Images are representative of 3 independent experiments.