Table 1.
Primary antibody | Made in | Supplier | Part number | Dilution | Secondary antibody (dilution) * |
---|---|---|---|---|---|
Anti-mGluR1 | Rb | Millipore | 07-617 | 1:500 | Dk anti-Rb IRDye 700 (1:20,000) |
Anti-mGluR5 | Ms | Millipore | MABN540 | 1:500 | Dk anti-Ms IRDye 800 (1:15,000) |
Anti-mGluR2/3 | Rb | Millipore | 06-676 | 1:1000 | Dk anti-Rb IRDye 700 (1:20,000) |
Anti-mGluR4 | Rb | Millipore | AB15097 | 1:1000 | Dk anti-Rb IRDye 800 (1:15,000) |
Anti-mGluR7 | Gt | Abcam | ab85343 | 1:1000 | Dk anti-Gt IRDye 700 (1:20,000) |
Anti-mGluR8 | Gt | Santa Cruz Biotechnology | sc-30300 | 1:500 | Dk anti-Gt IRDye 800 (1:15,000) |
Anti-α-tubulin | Ck | Sigma-Aldrich | SAB3500023 | 1:2000 | Dk anti-Ck IRDye 700 (1:20,000) |
Ck = chicken, Dk = donkey, Gt = goat, Ms = mouse, Rb = rabbit.
All secondary antibodies were purchased from LI-COR Bioscience.