Table 3.
Summary of statistical analyses
| Location | Description | Data structure | Type of test | Statistical value |
|---|---|---|---|---|
| a | Fig. 1B–E | Normal (Gaussian distribution) at the end point; number (n) of biological experiments = 4 with three technical replicates | Two-way repeated-measures ANOVA after Bonferroni correction | Number (#) of MNs : p < 0.001, F = 13.63, DFn = 1; Neurite length per cell: p < 0.01, F = 5.27, DFn = 1; # of Nodes per cell : p < 0.001, F= 30.57, DFn = 1; Cell body size per cell : p < 0.001, F = 11.54, DFn = 1 |
| Unpaired two-tailed t-test at the end point | # of MNs : p < 0.001; Neurite length per cell: p < 0.01; # of Nodes per cell : p < 0.001; Cell body size per cell : p < 0.001 | |||
| b | Fig. 1F | Normal, n = 4 | Unpaired two-tailed t-test | p < 0.05 |
| c | Fig. 1G | Normal, n = 4 | Unpaired two-tailed t-test | # of MNs: p < 0.01, Neurite length per cell: p < 0.05; # of Nodes per cell: p < 0.0001, Cell body size: p < 0.01 |
| d | Fig. 2C | Normal, n = 5 | Unpaired two-tailed t-test | TF+: p < 0.001, TF addback at day 6: p < 0.05 |
| e | Fig. 2D, E | Normal at the end point; n = 5 | Two-way repeated-measures ANOVA after Bonferroni correction | Neurite length per cell: p < 0.01, F = 6.555, DFn = 4; # of Nodes per cell: p < 0.05, F = 3.356, DFn = 4 |
| Unpaired two-tailed t-test at the end point | Neurite length per cell: TF addback at day 6, day 7, and day 8: p < 0.001, respectively; # of Nodes per cell: TF addback at day 6, day 7: p < 0.001; TF addback at day 8: p < 0.01 | |||
| f | Fig. 2G | Normal, n = 5 | Unpaired two-tailed t-test | TF+ and TF+/Ken 5µM: p < 0.001; TF–/2.5µM and TF–/5µM: p < 0.05 |
| g | Fig. 2H, I | Normal at the end point; n = 5 | Two-way repeated-measures ANOVA after Bonferroni correction | Neurite length per cell: p < 0.05, F = 4.01, DFn = 4; # of Nodes per cell: not significant |
| Unpaired two-tailed t-test at the end point | Neurite length per cell: TF+ and TF+/Ken 5µM: p < 0.001; # of Nodes per cell: TF+: p < 0.001,; TF+/Ken5 µM: p < 0.01 | |||
| h | Fig. 3D | Normal at the end point; n = 5 | Two-way repeated-measures ANOVA correction after Bonferroni correction | Fold change of MNs with 4 or more nodes :p < 0.05, F = 3.949, DFn = 2 |
| Unpaired two-tailed t-test at the end point | p < 0.05 | |||
| i | Fig. 4C | Normal, n = 5 | Unpaired two-tailed t-test | TF+: p < 0.001, TF addback at day 6: p < 0.001 |
| j | Fig. 4D | Normal, n = 5 | Unpaired two-tailed t-test | TF+: p < 0.01, TF+/Ken 5 µM: p < 0.01 |
| k | Fig. 5C | Normal, n = 5 | Unpaired two-tailed t-test | TF+: p < 0.001, TF addback at day 6: p < 0.001, TF addback at day 7: p < 0.05, addback at day 8: p < 0.05, |
| l | Fig. 5D | Normal, n = 5 | Unpaired two-tailed t-test | TF+: p < 0.001, TF addback at day6: p < 0.001,; TF addback at day7: p < 0.05, addback at day8: p < 0.05 |
| m | Fig. 5E | Normal, n = 5 | Unpaired two-tailed t-test | TF+: p < 0.001, TF+/Ken 5 µM: p < 0.001,; TF–/Ken 2.5 µM: p < 0.01, TF–/Ken 5µM: p < 0.01 |
| n | Fig. 5F | Normal, n = 5 | Unpaired two-tailed t-test | TF+: p < 0.01, TF+/Ken 5 µM: p < 0.01 |
| o | Fig. 6C | Normal, n = 5 | Unpaired two-tailed t-test | TF addback at day 6, day 7, and day 8: p < 0.001, respectively |
| p | Fig. 6D | Normal, n = 5 | Unpaired two-tailed t-test | TF addback at day 6, day 7, and day 8: p < 0.001, respectively |