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. Author manuscript; available in PMC: 2018 Jun 29.
Published in final edited form as: Cancer Cell. 2016 Apr 11;29(4):494–507. doi: 10.1016/j.ccell.2016.03.006

Figure 5. LUBAC recruitment to the CBM complex in ABC DLBCL depends on cIAP1/2.

Figure 5

(A) SHARPIN IPs or total lysates of the indicated DLBCL lines treated with birinapant (5 µM) for 24 hours were immunoblotted for the indicated proteins. (B) Total SDS lysates or after biotin-labeled K63-specific TUBE binding and streptavidin purification from the indicated DLBCL lines were analyzed by immunoblotting. (C) SHARPIN IPs of DLBCL lines treated with birinapant (5 µM) for 24 hours or total lysates were immunoblotted for the indicated proteins. (D) Lysates prepared using 1% SDS from HBL1 cells treated with birinapant (5 µM) or ibrutinib (10 nM) for 24 hours were diluted and then subjected to IP. IPs or total lysates were immunoblotted for the indicated proteins. (E) Lysates prepared using 1% SDS from ABC DLBCL cells treated with birinapant (5 µM) for 24 hours were diluted and subjected to IP. IPs or total lysates were immunoblotted for the indicated proteins. See also Figure S5.