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. Author manuscript; available in PMC: 2019 Jul 1.
Published in final edited form as: Heart Rhythm. 2018 Feb 16;15(7):1072–1080. doi: 10.1016/j.hrthm.2018.02.018

Figure 4. HuR protects SCN5A mRNA from decay.

Figure 4

(A) Overexpression of HuR increased SCN5A mRNA stability. Transcription of cells with the TetOn pTRIPz-HuR construct was blocked by addition of actinomycin D (Act D) (5 μg/mL) after 48 hours of doxycycline induction. Cells were harvested at different time points after Act D addition and the levels of SCN5A mRNA were measured using qRT-PCR. The graph shows the percentage of remaining SCN5A mRNA levels in relation to β-actin mRNA compared with the levels of normalized mRNA measured immediately before addition of Act D. The initial mRNA levels were set to 100%. (B) HuR-specific siRNA decreased the mRNA stability of SCN5A. Transcription was blocked with addition of Act D as described above 48 hours after transfection of scrambled (Scrmbl) or HuR-specific siRNA. The remaining SCN5A mRNA levels were measured as described above. Error bars represent mean ± SD. *P<0.05.