Table 2.
Time points (weeks) | 4 °C |
RT |
30 °C |
|||||
---|---|---|---|---|---|---|---|---|
Mean1 | P-value3 | Mean | P-value | Mean | P-value | |||
Average huDNA copy number | 0–8 weeks | 0 | 1034 | 0.41 | 1132 | 0.61 | 3923 | 0.37 |
2 | 1416 | 1012 | 3208 | |||||
4 | 3543 | 912 | 1078 | |||||
8 | 1013 | 2013 | 469 | |||||
0–32 weeks | 0 | 3489 | 0.03 | 1635 | 0.54 | 1216 | 0.54 | |
16 | 680 | 1184 | 1222 | |||||
32 | 1005 | 975 | 804 | |||||
2Relative number of 100 bp. fragments | 0–8 weeks | 0 | 678 | 0.44 | 609 | 0.91 | 1157 | 0.08 |
2 | 744 | 596 | 698 | |||||
4 | 669 | 629 | 715 | |||||
8 | 573 | 655 | 519 | |||||
0–32 weeks | 0 | 906 | 0.05 | 830 | 0.05 | 839 | 0.00 | |
16 | 618 | 606 | 595 | |||||
32 | 560 | 489 | 463 | |||||
Relative number of 200 bp. fragments | 0–8 weeks | 0 | 252 | 0.79 | 190 | 0.678 | 748 | 0.03 |
2 | 250 | 246 | 265 | |||||
4 | 324 | 159 | 375 | |||||
8 | 193 | 284 | 88 | |||||
0–32 weeks | 0 | 1163 | 0.09 | 485 | 0.24 | 299 | 0.03 | |
16 | 111 | 181 | 209 | |||||
32 | 214 | 188 | 108 | |||||
Relative number of 300 bp. fragments | 0–8 weeks | 0 | 1529 | 0.36 | 1387 | 0.616 | 3510 | 0.03 |
2 | 1511 | 1307 | 1662 | |||||
4 | 11125 | 1033 | 1604 | |||||
8 | 1211 | 1826 | 642 | |||||
0–32 weeks | 0 | 5018 | 0.04 | 2059 | 0.467 | 1811 | 0.69 | |
16 | 762 | 1379 | 2608 | |||||
32 | 1456 | 1280 | 1491 | |||||
Relative number of 400 bp. Fragments | 0–8 weeks | 0 | 1253 | 0.35 | 1802 | 0.884 | 2990 | 0.44 |
2 | 1407 | 1660 | 11793 | |||||
4 | 2453 | 1577 | 1484 | |||||
8 | 1609 | 2193 | 683 | |||||
0–32 weeks | 0 | 4968 | 0.03 | 1905 | 0.90 | 1408 | 0.51 | |
16 | 1399 | 1730 | 1292 | |||||
32 | 1505 | 1594 | 1006 | |||||
Relative number of 500 bp. fragments | 0–8 weeks | 0 | 1459 | 0.51 | 1673 | 0.554 | 11211 | 0.25 |
2 | 3168 | 1261 | 1623 | |||||
4 | 3158 | 1168 | 1267 | |||||
8 | 1480 | 5129 | 419 | |||||
0–32 weeks | 0 | 5389 | 0.05 | 2913 | 0.59 | 1735 | 0.50 | |
16 | 509 | 2025 | 1424 | |||||
32 | 1304 | 1337 | 956 |
Average amplifiable copy numbers
Overall and relative composition of the sample evaluated as proportion of 100, 200, 300, 400 and 500 bp DNA fragments.
p=value calculated using the one-way ANOVA test