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. 2018 Jun 29;8:9814. doi: 10.1038/s41598-018-28249-5

Figure 1.

Figure 1

mRNA and protein expression of lactate transporters. Satellite cells were cultured and differentiated to myotubes. They were kept in normal differentiation medium (basal, 5.5 mM glucose) or exposed to lactic acid (4 mM or 10 mM replacing glucose) for 24 h before total cell RNA and protein content were isolated. (a) mRNA expressions of SLC16A1 (MCT1) and SLC16A4 (MCT4). All values were corrected for the housekeeping gene acidic ribosomal phosphoprotein P0 (RPLP0), and presented as means ± SEM relative to basal (n = 5). (b,c) Protein expressions of MCT1–4. (b) One representative immunoblot. (c) Quantified immunoblots relative to basal. All values were corrected for the housekeeping control α-tubulin, and data are presented as means ± SEM relative to basal (n = 5). Dividing lines delineate blots from different gels. The samples derive from the same experiment, and all blots were processed in parallel. Full-length blots are presented in Supplementary Fig. 1. *Statistically significant vs. basal (p < 0.05, paired Student’s t-test).