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. 2018 Jun 29;8:9814. doi: 10.1038/s41598-018-28249-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Incorporation of lactic acid into cellular lipids. Myotubes were incubated with [¹⁴C(U)]lactic acid (1 µCi/ml, 20 mM) for the last 24 h of the differentiation period before total cell lipids were extracted and separated by thin layer chromatography. Incorporation into cellular lipids calculated as % of total lipid content (57.9 ± 29.5 nmol/mg protein). Data are presented as means ± SEM (n = 3). PL, phospholipids; DAG, diacylglycerol; FFA, free fatty acids; TAG, triacylglycerol; CE, cholesteryl ester. *Statistically significant vs. basal (p < 0.05, paired Student’s t-test).