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. 2018 Jun 29;8:9821. doi: 10.1038/s41598-018-28183-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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No TLR4 alterations in retina and optic nerve. (A) Retinal cross-sections were labeled with an anti-TLR4 antibody (red) and DAPI (blue) at 3, 7, and 14 days. (B) No alterations in the number of TLR4⁺ cells were observed in the S100 retinas at all points in time (p > 0.05). (C) Also, no altered Tlr4 mRNA expression could be noted at all points in time via qRT-PCR (p > 0.05). (D) To determine TLR4 expression in optic nerves, sections were stained with anti-TLR4 (red) and DAPI (cell nuclei, blue). (E) After 3 days, no changes were seen regarding the TLR4 expression in the S100 animals (p > 0.05). Also, no alterations were detected later on (p > 0.05). Abbreviation: GCL = ganglion cell layer. Values are mean ± SEM for immunohistology and median ± quartile + maximum/minimum for qRT-PCR. Scale bars: 20 µm.