. 2018 Jun 29;8:9821. doi: 10.1038/s41598-018-28183-6

Table 3.

Oligonucleotide sequences.

Gene	Forward (F) and reverse (R) oligonucleotides	GenBank acc. no.	Amplicon size
Bax-F	gctggacactggacttcctc	NM_017059.2	127 bp
Bax-R	actccagccacaaagatggt	NM_017059.2	127 bp
Bcl-2-F	gtacctgaaccggcatctg	NM_016993.1	76 bp
Bcl-2-R	ggggccatatagttccacaa	NM_016993.1	76 bp
C3-F	tcgaaatccctcccaagtc	NM_016994.2	60 bp
C3-R	cgatcttcaaggggacaatg	NM_016994.2	60 bp
C5-F	tctcaggccaaagagagacc	XM001079130.4	73 bp
C5-R	acggtgtttgtatttagcagctt	XM001079130.4	73 bp
Cyclophilin-F	tgctggaccaaacacaaatg	M19553.1	88 bp
Cyclophilin-R	cttcccaaagaccacatgct	M19553.1	88 bp
Factor b-F	aagaaggcagaatgcagagc	NM_212466.3	66 bp
Factor b-R	ccagaactccccattttcaa	NM_212466.3	66 bp
Mbl-F	aggaccaaaaggccaaaaag	NM_012599.2	68 bp
Mbl-R	ccatatttgccagcttcacc	NM_012599.2	68 bp
Myd88-F	atgaactgaaggaccgcatc	XM_006244087.3	127 bp
Myd88-R	cccagttcctttgtctgtgg	XM_006244087.3	127 bp
NEMO-F	tctgaagaaatgccaacagc	AY392762.1	61 bp
NEMO-R	tgtcacctgagctttcacaga	AY392762.1	61 bp
Nfκb-F	ctggcagctcttctcaaagc	NM_001276711.1	70 bp
Nfκb-R	ccaggtcatagagaggctcaa	NM_001276711.1	70 bp
Pou4f1-F	ctccggaccttgagcttct	XM_008770931.2	60 bp
Pou4f1-R	tagaagggagagttaaacacagaaca	XM_008770931.2	60 bp
Tlr4-F	ccttgagaaagtggagaagtcc	NM_019178.1	61 bp
Tlr4-R	gctaagaaggcgatacaattcg	NM_019178.1	61 bp
β-actin-F	cccgcgagtacaaccttct	NM_031144.3	72 bp
β-actin-R	cgtcatccatggcgaact	NM_031144.3	72 bp

The listed oligonucleotide pairs were used in quantitative real-time PCR experiments, while β-actin and cyclophilin served as housekeeping genes. The predicted amplicon sizes are given. Abbreviations: F = forward, R = reverse, acc. no. = accession number, bp = base pair.