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. 2018 Jun 29;8:9841. doi: 10.1038/s41598-018-28153-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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qPCR analyses of 12 selected genes in gallnuts at different developmental stages (after aphids feeding). (A) Line with circle is the expression profile in gallnuts. TS-2, TS-4, TS-9, TS-10, TS-14, TS-15, TS-17 and TS-18 are the putative genes involved in tannin biosynthesis; PD-1 and PD-8 represent the putative genes related to plant defense; PHS-1 and PHS-2 represent the genes related to plant hormone synthesis. Horizontal axis indicates sample collection time (Jun. 21, Jul. 26, Aug. 8, Aug. 18 and Oct. 12, respectively). (B) The correlation between qRT-PCR and RPKM data.