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. 2018 Jun 29;8:9868. doi: 10.1038/s41598-018-28199-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Bacterial growth with exposure to colistin sulfate and SNPs obtained at each day. The amount of bacterial growth on solid media was observed over a range of colistin concentrations (0–128 μg/ml) for 2 cultures of Ecl8 over 8–10 days for C1 (A) and C2 (B). The horizontal black lines represent bacterial growth on different concentrations of colistin over a 5 day time period, then growth after colistin selection is removed for another 5 days or until colistin sensitivity was reached. The boxes below represent the SNPs identified in each culture at D3, D5 or D10 and the amount of pink fill represents the proportion of the population harboring the SNP and this number is displayed below.