Fig. 6. RSV treatment enhanced the periodontal regeneration and osteogenesis of P-PDLSC aggregates in vivo.

a Histological analysis of periodontal bone regeneration (denoted by B) and collagen bone matrix deposition (denoted by C) of PDLSC aggregates by HE (top) and Masson’s trichrome staining (bottom). b Immunohistochemistry analysis of the osteogenic marker genes bone sialoprotein (Bsp) and osteopontin (Opn). c–e Statistical analysis showed that P-PDLSC aggregates regenerated less bone and produced less collagen matrix in periodontal defects and demonstrated weaker osteogenic potential, which could be improved by RSV treatment. RSV was applied at 10 nM throughout the in vitro treatments. n = 6 per group. The data represent the mean ± SD. *P < 0.05