Fig. 5.

The change of EBNA1 expression induced by Hsp70 is not associated with the GAr domain, and Hsp70 promote the lytic replication of EBV in HONE1/Akata cells but PES inhibits this effect. HONE1/Akata cells were transfected with pEF-Flag-Hsp70 (a) or Hsp70 siRNA (b) for 24 h, followed by treatment with PES (20 or 40 μM) for 48 h. c HONE1/Akata and HeLa cells were transfected with pEF-Flag-Hsp70 for 24 h, followed by transfection with pSG5-EBNA1 or pSG5-EBNA1ΔGA into above cells for 48 h. d HONE1/Akata and HeLa cells were transfected with Hsp70 siRNA for 24 h, followed by transfection with pSG5-EBNA1 or pSG5-EBNA1ΔGA into above cells for 48 h. Whole-cell proteins above were extracted and subjected to western blotting. HONE1/Akata cells were induced by TPA and NaB for 4 h, and then transfected with pEF-Flag-Hsp70 (e) or Hsp70 siRNA (g), followed by treatment with PES beginning at 4 h after transfection. The intracellular genomic DNA were quantified by RT-PCR with EBNA1 primers. Each sample was normalized to the amount of the GAPDH gene. f,h Whole-cell proteins above were extracted and analyzed using western blotting (**P < 0.01, ***P < 0.001)