Figure 5.

In vivo images and serial block face electron microscopy (SBF-SEM) sections obtained during IA mediated remodeling of zebrafish CVP. (A) In vivo images of CVP from zebrafish embryo and the corresponding region of interest (A₁) in the GFP image of intussusceptive pillars (indicated by asterisk) shown at higher magnification. (B-B₃) Representative SBF-SEM images showing a set of 4 pillars found in the distal region of the remodeling CVP (series of sections comprising 500 slices, each with 50 nm thickness). (C) Three dimensional reconstruction of SBF-SEM sections illustrates the volume of intussusceptive pillars. Erythrocytes have been removed in order to demonstrate the spatial orientation of three pillars. For further information, see Supplementary Movies S5 and S6.