Fig. 5.

Cellular phenotypes in the fly eye due to knockdown of individual 16p11.2 homologs. a Representative brightfield adult eye images (scale bar = 50 µm) and confocal images of pupal eye (scale bar = 5 µm) and larval eye discs (scale bar = 30 µm), stained with anti-Dlg and anti-pH3 respectively, of select 16p11.2 homologs illustrate defects in cell proliferation caused by eye-specific knockdown of these homologs. b Table summarizing the cellular defects observed in the pupal eye of 16p11.2 homologs. “+” symbols indicate the severity of the observed cellular defects. c Box plot of Flynotyper scores for knockdown of 13 homologs of 16p11.2 genes with GMR-GAL4 > Dicer2 (n = 7–19, *p < 0.05, Mann–Whitney test). FAM57B^CG17841 knockdown displayed pupal lethality with Dicer2, and therefore the effect of gene knockdown in further experiments was tested without Dicer2. d Box plot of photoreceptor cell count in the pupal eyes of 16p11.2 knockdown flies (n = 59–80, *p < 0.05, Mann–Whitney test). e Box plot of pH3-positive cell count in the larval eyes of 16p11.2 knockdown flies (n = 6–11, *p < 0.05, Mann–Whitney test). f Box plot of adult eye area in 16p11.2 one-hit knockdown models (n = 5-13, *p < 0.05, Mann–Whitney test). All boxplots indicate median (center line), 25th and 75th percentiles (bounds of box), and minimum and maximum (whiskers)