Fig. 2.

Simulated data generated across physiological ranges exhibit varying degrees of noise and statistical variance. A) Histogram of values of transit times determined via correlation analysis from 640 measurements from scanning FCS simulations with 1.0 μm²/s (grey), 0.5 μm²/s (turquoise), 0.2 μm²/s (green) and 0.05 μm²/s (blue) diffusion rates, representing 10x carpets from each simulation. (Inset) The same data but with natural logarithm of transit time values. B) Correlation functions from 0.2 μm²/s diffusion simulations (black dots) with parameterised fits (blue lines) with different forms of noise annotated on the different parts of the curve, and (lower panel) residuals from curve fitting (grey lines). (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)