Fig. 5.

Photobleaching dramatically affects transit time measurements but can be restored through suitable corrections. A,B) Intensity time-series from a simulated experiment (D = 0.5 μm²/s, duration 3000 ms), integrated over all pixels from simulation without (A) and with photobleaching (B). (C) Respective correlation curves from the simulation without (black line) and with photobleaching (green line, analyzed in the conventional way), and in the latter case with correction method 1 (red line) and correction method 2 (blue line). D) Whisker plots of values of transit times determined from 64 simulated measurements generated with and without photobleaching, original data and analysis without correction and with correction methods 1 and 2, as labeled. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)