Table 1.
Strains and plasmids used in this study
| Genotypea | |
|---|---|
| Strains | |
| K1534* | MATa ade2-1 trp1-1 can1-100 leu2-3, 112 his3-11, 15 ura3 ssd1 bar1∷hisG (Irniger and Nasmyth, 1997) |
| K4438* | K1534 cdc16-123 (Irniger and Nasmyth, 1997) |
| MAY2063 | MATa ade2-101 lys2-801 his3-Δ200 leu2-3, 112 ura3-52 cin8∷URA3 |
| MAY2422 | MATa lys2-801 his3-Δ200 leu2-3, 112 ura3-52 cyh2R |
| MAY4751 | MATa ade2-101 his3-Δ200 leu2-3, 112 ura3-52 cin8∷CIN8-3HA cdc15-2 |
| MAY4822 | MATa lys2-801 his3-Δ200 leu2-3, 112 ura3-52 cin8∷CIN8-3HA |
| MAY4823 | MATa ade2-101 lys2-801 his3-Δ200 leu2-3, 112 ura3-52 cin8∷CIN8-3HA |
| MAY5017 | MATa lys2-801 his3-Δ200 leu2-3, 112 ura3-52 cdc20-1 cin8∷CIN8-3HA |
| MAY5353* | K1534 cdh1∷KANR |
| MAY5402* | MATa ade2-1 trp1-1 leu2-3, 112 his3 ura3 bar1∷hisG cdc20-1 |
| MAY5466 | MAY4822 cdh1∷KANR |
| MAY5590 | MATa ade2-101 lys2-801 his3-Δ200 leu2-3, 112 ura3-52 cyh2 cin8∷HIS3 kip1∷HIS3 pTK97 |
| MAY6752 | MATα lys2-801 his3-Δ200 leu2-3, 112 ura3-52 cyh2 cin8∷HIS3 dyn1-Δ3∷HIS3 pEH24 |
| MAY6810* | K1534 clb2∷URA3 |
| MAY6812* | K1534 clb2∷URA3 cdh1∷KANR |
| Plasmids | |
| pEH24 | CIN8 URA3 CYH2 CEN |
| pEH55 | PGAL1 > CIN8-3HA LEU2 CEN |
| pEH70 | PGAL1 > 6myc-CIN8 LEU2 CEN |
| pEH87 | PGAL1 > ΔN70-CIN8-3HA LEU2 CEN |
| pEH113 | CIN8 LEU2 CEN |
| pEH117 | 6myc-CIN8-1031 LEU2 2μ |
| pEH136 | PGALS > CIN8 LEU2 CEN |
| pEH157 | 6myc-SV40NLS-CIN8-1031 LEU2 2μ |
| pEH170 | PGAL1 > 6myc-SV40NLS-CIN8 LEU2 CEN |
| pEH287 | PGALS > CIN8 HIS3 CEN |
| pEH289 | PGAL1 > GAL4BD-cin8 LEU2 CEN |
| pEH290 | PGAL1 > GAL4BD-cin8-Δ996-1004 LEU2 CEN |
| pEH291 | PGAL1 > GAL4BD-cin8-alaKEN LEU2 CEN |
| pEH292 | PGAL1 > GAL4BD LEU2 CEN |
| pTK97 | KIP1 URA3 CEN |
| pTK138 | 6myc-CIN8 LEU2 2μ |
a
Strains marked with an asterisk are derivatives of W303. All other strains are derivatives of S288C.