Table 2.

Cells expressing stable Cin8p accumulate mitotic cells with no spindle

Relevant genotypeb	Large-budded (mitotic) cells witha
	No spindle	Short spindle	Anaphase spindle
	(%)	(%)	(%)	(n)
CIN8	21	47	32	383
cin8-KED	42	38	20	309
cin8-Δ996-1004	36	42	22	480

^a The indicated strains were grown to midlog phase, fixed, and stained for microtubules and DNA. Large-budded cells were selected microscopically, and their microtubules were observed by indirect immunofluorescence. Short spindles were defined as a bright bar of microtubule staining approximately half the diameter of the mother cell body (approximately 2–3 μm). Anaphase spindles were defined as a line of microtubule staining extending into both mother and bud and from half to two cell body diameters in length. “No spindle” cells contained no clearly observable bipolar structure but possessed clear arrays of microtubules in either one or both cell bodies. 

^b cin8Δ strain (MAY2063) carrying a low-copy CEN plasmid with CIN8, cin8-KED, or cin8-Δ996-1004.