Table 1.
Body composition and blood biochemistry in female AZIP lipodystrophic mice (n = 8–10) and wild type littermate controls (n = 8–10) whilst being fed a regular low fat chow-(LFD) or high fat diet (HFD). Blood was taken in the fed state for all parameters except for glucose and insulin that were measured in both the fed state and after a 5 h fast.
| Wildtype |
AZIP |
|||
|---|---|---|---|---|
| LFD | HFD | LFD | HFD | |
| Body weight (g) | 25.9 ± 1.0 | 27.5 ± 1.1*** | 26.5 ± 0.8 | 26.8 ± 0.7 |
| Fat mass (%) | 20.9 ± 2.7 | 29.3 ± 2.2*** | 5.5 ± 0.8### | 7.8 ± 0.3###* |
| Glucose (mmol/l) | ||||
| fed | 10.5 ± 0.3 | 11.3 ± 0.3 | 25.6 ± 4.3### | 34.6 ± 0.7###* |
| fasted | 7.9 ± 0.2&&& | 7.9 ± 1.1& | 13.4 ± 4.2##& | 32.5 ± 1.3###**& |
| Insulin (μg/l) | ||||
| fed | 1.1 ± 0.3 | 1.2 ± 0.2 | 71.5 ± 21.0### | 133.1 ± 3.6###** |
| fasted | 0.7 ± 0.1& | 0.8 ± 0.2& | 16.7 ± 2.7###&& | 27.6 ± 6.5###**&&& |
| TG (mmol/l) | 3.8 ± 0.4 | 2.1 ± 0.1* | 14.1 ± 2.8### | 34.2 ± 5.8##** |
| Urine glucose (mmol/l) | 3.2 ± 0.3 | 4.3 ± 0.2 | 406.7 ± 19.6### | 529.6 ± 17.5**,### |
| CHO energy loss (kJ/mouse/day) | 0.03 ± 0.00 | 0.03 ± 0.00 | 18.10 ± 1.85### | 18.03 ± 0.36### |
| Liver weight (g) | 1.31 ± 0.08 | 1.35 ± 0.11 | 3.58 ± 0.15## | 4.52 ± 0.19## |
| Soleus weight (mg) | n.d. | 6.33 ± 0.30 | n.d. | 5.42 ± 0.73 |
| EDL weight (mg) | n.d. | 8.83 ± 0.44 | n.d. | 8.50 ± 0.31 |
| Gas. Weight (mg) | n.d. | 113.26 ± 10.6 | n.d. | 122.73 ± 4.12 |
TG, triglyceride; CHO, carbohydrate; EDL, extensor digitorum longus; Gas., gastrocnemius.
*p < 0.05, **p < 0.01, ***p < 0.001 compared to LFD (t-test).
#p < 0.05, ##p < 0.01, ###p < 0.001 compared to WT (t-test).
&p < 0.05, &&p < 0.01, &&&p < 0.001 compared to the fed state (t-test).