Table 1.

In vitro antagonistic activities of C-terminal FGF21 and FGF19 Ala-scan peptides.

IC₅₀ values (presented in μM, mean ± SD, n=3) and fold-changes in antagonistic activity of Ala-scan mutants vs. respective native peptides determined by inhibition of FGF21-induced pERK signaling in 293/KLB cells. The peptide sequences are aligned to maximize sequence identity (157-181 in FGF21, vs. 169-194 in FGF19) with native amino acids shown in one letter code. The alanine substitutions of significantly lower potency compared to native peptide (4x or more) are highlighted in red bold font. NC denotes where IC₅₀ values were Not Calculated due to negligible activity at the tested concentrations.