Figure 3.

Cytomorphology of pancreatic islets (PIs) from WT-SC, WT-GDFE and SGLT1^−/−-GDFE mice. Individual measurements of β- and α-cell proportions are indicated as means ± SD. (A) Representative pictures of PIs with atypical and typical cytomorphology. The PIs were stained with antibodies against insulin for β-cells (INS, green), glucagon for α-cells (GCG, red), and DAPI (blue). Scale bar = 100 μm. B: Proportion of PIs showing an atypical cytomorphology (n = 6). Per male animal, 4–6 sections from different stacks were analyzed. (C,D) Proportion of β- (C) or α-cells (D) related to total counted cells per islet (n = 6). 4–6 sections of different stacks were analyzed. *P < 0.05, **P < 0.01, ***P < 0.001, ns = not significant ANOVA with posthoc Tukey comparison.