Figure 1.

Osteoblastic Y1 receptor signaling modulates insulin secretion and insulin action via osteoglycin. Scatter plots showing osteoblast cells isolated from (A) non-GFP expressing mice and (B) GFP expressing mice showing GFP signal, forward scatter (FSC) and cell count. Green GFP positive (GFP POS) gate represents the purified osteoblast cells that are selected for further analysis. (C) Representative photomicrograph showing GFP positive osteoblasts. qPCR of osteoglycin mRNA levels in (D) wildtype (WT) and Y1^−/− osteoblastic cells and (E) WT and Y1^lox/lox3.6Cre femora. (F) qPCR of Ins1 and Ins2 mRNA levels in MIN6 cells with different doses of osteoglycin. (G) Insulin secretion from MIN6 cells during a glucose stimulated insulin secretion test with or without osteoglycin (1 μg/mL). Data are means ± SEM of at least 6 per group, * = p < 0.05 as indicated. Scale bar represents 5 μm.