Table 3.

Recent advances in microalgae employed for the production of biofuel

Type of model or algorithm	Target product	Model-based target: knockout gene A or overexpress gene B	References
Dynamic flux balance analysis	Ethanol	Ethanol production pathway after 20 h	[163]
Genome-scale metabolic model	Prediction of growth rate with respect to photosynthetic quotient for ethanol and butanol production	Double reaction knock out of hydrolyase and dehydrogenase	[164]
In vivo carbon flux analysis	Homolanthionine	Deletion of methionine and cysteine biosynthesis repressor protein	[165]
Metabolic flux analysis	Hydrogen production	Deletion and addition of GAP1 and dehydrogenase, respectively	[166]
Metabolic flux analysis	Astaxanthin synthesis	–	[167]
Metabolic flux analysis	Fixed more CO2 and had a higher biomass yield	Alternate pathway for isoleucine synthesis (via citramalate synthase, CimA	[168]
Metabolic net fluxes	Hydrogen production	Disruption of hydrogenase and poly-β-hydroxybutyrate synthase	[169]