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. 2018 Jun 19;114(12):2833–2843. doi: 10.1016/j.bpj.2018.04.046

Figure 3.

Figure 3

Sedimentation-velocity analysis of BjPutA after treatment with active site ligands or NPPG. (A) The distribution of sedimentation coefficients for untreated BjPutA (black), BjPutA supplemented with active site ligands (10 mM THFA and 1 mM NAD+; blue), NPPG-inactivated BjPutA (red), dithionite-reduced BjPutA (purple), and the previously reported BjPutA hot spot mutant dimer (green) (16) are shown. (B) The distribution of sedimentation coefficients for untreated BjPutA (black), BjPutA supplemented with 200 mM proline (magenta), BjPutA supplemented with 1 M proline (orange), and the previously reported BjPutA hot spot mutant dimer (green) (16) are shown. All experiments were run at a protein concentration of 5 mg mL−1 (23 μM based on dimer Mr). To see this figure in color, go online.