Figure 4.
SAXS analysis of oxidized and NPPG-inactivated (reduced) BjPutA. (A) The composite experimental SAXS curve of untreated BjPutA obtained from SEC-MALS-SAXS is shown in open circles. The red curve was calculated from the crystallographic BjPutA tetramer (PDB: 3HAZ) using FoXS (χ: 1.1). The top right inset shows the Guinier plot and the calculated Rg. Also shown is a DAMMIF shape reconstruction superimposed with the crystallographic tetramer. (B) The composite experimental SAXS curve of NPPG-inactivated BjPutA obtained from SEC-MALS-SAXS is shown in open circles. The red curve was calculated from the crystallographic BjPutA dimer (PDB: 3HAZ) using FoXS (χ: 1.7). The cyan curve shows the two-body fit composed of 93%:7% BjPutA dimer:tetramer generated by MultiFoXS (χ: 1.3). The top right inset shows the Guinier plot and the calculated Rg. Also shown is a DAMMIF shape reconstruction superimposed with the crystallographic dimer. (C) Experimental distance distribution functions for untreated BjPutA (red) and NPPG-inactivated BjPutA (blue) are shown. To see this figure in color, go online.