Figure 2.

Analysis of 2D trajectories of 40 nm AuNP-labeled membrane protein probes. (a) Displacement in x (in direction of neurite propagation, black) and y (perpendicular to direction of neurite propagation, red) for a bead tracked at 2 kHz is shown. (b) The same scenario is shown for a bead tracked at 40 kHz. (c) Time-dependent one-dimensional diffusion coefficient (x dimension) plotted against the time-lag used for analysis. Shown are plots for membrane proteins differing in their cytosolic domains and membrane anchors. TM = L-YFP-GT46, TMX = L-YFP-GTmHoneydew46, GPI-GFP = glycosylphosphatidylinositol-anchored green fluorescent protein. To see this figure in color, go online.