Table 1.
Fitted and Calculated Gating Parameters
| QON | ITAIL | ITAIL-QON | ||||||
|---|---|---|---|---|---|---|---|---|
| Construct | Parameter | Mean | SE | n | Mean | SE | n | Difference |
| VM (mV) | −38.9 | 0.8 | 6 | −31.6 | 0.8 | 6 | 7.3a | |
| W207A-N214R | V0.5 (mV) | −40.0 | 1.1 | 6 | −31.4 | 0.6 | 6 | 8.6b |
| zG (e0) | 2.80 | 0.06 | 6 | 3.21 | 0.11 | 6 | 0.41NS | |
| VM (mV) | −24.1 | 0.3 | 12 | −11.3 | 0.4 | 12 | 12.8c | |
| W207A-N214R-ΔC | V0.5 (mV) | −24.6 | 0.3 | 12 | −15.7 | 0.4 | 12 | 8.9d |
| zG (e0) | 1.75e | 0.01 | 12 | 1.52f | 0.02 | 12 | −0.23g | |
| VM (mV) | 17.8 | 0.8 | 3 | 25.7 | 1.6 | 3 | 8.0NS | |
| R205A-N214R | V0.5 (mV) | 25.1 | 2.3 | 3 | 26.4 | 1.3 | 3 | 1.3NS |
| zG (e0) | 2.14h | 0.12 | 3 | 1.89i | 0.07 | 3 | −0.25NS | |
Data represent calculated VM or Boltzmann-fitted V0.5 and zδ parameters for QON-V and ITAIL-V relations measured at pHO 6.5 in the indicated number of cells.
a
W207A-N214R: ITAIL VM vs. QON VM, p = 0.03.
b
W207A-N214R: ITAIL V0.5 vs. QON V0.5, p = 0.02.
c
W207A-N214R-ΔC: ITAIL VM vs. QON VM, p = 1.6 × 10−7.
d
W207A-N214R-ΔC: ITAIL V0.5 vs. QON V0.5, p = 2.2 × 10−5.
e
QONzG: W207A-N214R vs. W207A-N214R-ΔC, p = 2.3 × 10−7.
f
ITAILzG: W207A-N214R vs. W207A-N214R-ΔC, p = 5.6 × 10−7.
g
W207A-N214R-ΔC: ITAILzG vs. QONzG, p = 0.01.
h
QONzG: W207A-N214R vs. R205A-N214R, p = 0.01.
i
ITAILzG: W207A-N214R vs. R205A-N214R, p = 0.02.