Figure 2. Allelic discrimination of SNP Amplifluor-like markers KIZ3 (A) and KIZ4 (B), designed for the BvSP2 and BvSE2 genes encoding chitinases, Class IV and Class III, respectively, among sugar beet breeding lines.

Red and blue dots indicate automatic SNP calls for homozygotes in Allele 1 (BvSP2-a₁a₁; BvSE2-a₂a₂) and Allele 2 (BvSP2-b₁b; BvSE2-b₂b₂), respectively, while green dots indicate heterozygotes (BvSP2-a₁b₁; BvSE2-a₂b₂) or mixed genotypes. Black squares show NTC (No Template Control). X- and Y-axes show Relative amplification units, ΔRn, for FAM and VIC fluorescence signals, respectively, as determined by the qPCR instrument.