Figure 3.

Rapamycin does not destabilize mRNAs in the presence of a rapamycin-resistant allele of the TOR1 kinase. The stability of the MFA2M3pG mRNA in a tor1Δ strain carrying either a plasmid bearing the WT TOR1 gene (TOR1) or the rapamycin-resistant TOR1–1 allele (TOR1–1) after treatment with rapamycin (+ rapamycin) or with vehicle alone (− rapamycin) for 210 min. The numbers above each lane indicate the number of minutes after transcription was repressed. The half-life values are the mean and SD of at least three experiments for each strain.