Figure 6.

Diauxic shift and rapamycin treatment cause the accumulation of a 5′-to-3′ decay intermediate without changing the rate of 3′-to-5′ decay. Analysis of the full-length MFA2M3pG mRNA and its decay fragment at log phase, diauxic shift, or in a tor1Δ strain carrying either the WT TOR1 gene or the rapamycin-resistant TOR1–1 allele after treatment with rapamycin (+ R) or vehicle alone (− R) for 210 min is shown. The MFA2M3pG mRNA and the decay fragment were detected with oRP127. Full-length and fragment species are indicated on the right. The ratio of the fragment to the full-length mRNA (F/FL) is indicated below each lane. The membrane was stripped and rehybridized with oRP100 to detect the 7S RNA as a loading control (bottom panel, 7S).