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. 2018 Jul 1;20:13. doi: 10.1186/s12575-018-0078-5

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Comparison of antigen retrieval methods in cancer cell-spiked human bone marrow. Permeabilization using 0.5% Triton X-100 compared to heat-mediated antigen retrieval using Citrate-based Antigen Retrieval Solution, Target Retrieval Solution, and EDTA solution. White blood cell (WBC) staining represents a cocktail of mouse WBC antibodies (CD45, CD14, CD34, and CD66b) detected by one anti-mouse secondary antibody. Filled white arrowheads point to true positive staining. Scale bar = 50 μm