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. 2018 Jul 1;20:13. doi: 10.1186/s12575-018-0078-5

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Optimization of secondary antibody application in human bone marrow. A) Comparison of different dilutions of whole secondary antibody and F(ab) fragment secondary antibody in primary unstained bone marrow. Goat anti-rabbit secondary antibodies for all three fluorophores were applied. Scale bar = 50 μm