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. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: Cancer Lett. 2018 May 30;431:142–149. doi: 10.1016/j.canlet.2018.05.047

Fig. 4. The ex vivo hypoxic tumor cells maintain increased survival against DNA damaging agents under the conventional culture conditions.

Fig. 4

The sorted hypoxic (EGFP+) and non-hypoxic (EGFP−) tumor cells were maintained under the ambient condition as described in Figure 3. (A) The sorted ex vivo cells were treated with Bleomycin or Etoposide at the indicated doses for 24 hours and then plated for clonogenic assay (n= 6, ****p<0.0001, ***p=0.0003). (B, C, D) The sorted tumor cells were treated with 6 μM Etoposide for 5 hours. 53BP1+ nuclear foci were stained and scored in five (n=5) random microscopic fields (B). Enzymatic cleavage of caspase 3 (C) and PARP (D) were analyzed by Western Blot using specific antibodies.

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