Characterization of a human squamous cell carcinoma cell line (A431) over-expressing human PPARβ/δ or PPARγ. (A) Representative photomicrographs of A431 cells, A431-Migr1 control cells (Migr1), A431-Migr1-hPPARβ/δ cells (hPPARβ/δ), and A431-Migr1-hPPARγ cells (hPPARγ) examined by fluorescent microscopy (upper panels) or light microscopy (lower panels). qPCR analysis for mRNA expression of (B) PPARβ/δ or (C) PPARγ in the A431 cell lines, normalized to GAPDH mRNA. (D) Western blot analysis of PPARβ/δ or PPARγ in the A431 cell lines, normalized to ACTIN expression. +positive control: cell lysate from COS-1 cells transfected with hPPARβ/δ or hPPARγ expression vector. qPCR analysis of ANGPTL4 mRNA in response to (E) the PPARβ/δ ligand GW0742 for 8 h or (F) the PPARγ ligand rosiglitazone (Rosi) for 24 h, normalized to the GAPDH mRNA. Data represents triplicate independent sample means ± S.E.M.. Values with different letters are significantly different (p ≤ 0.05).