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. Author manuscript; available in PMC: 2019 Jul 1.
Published in final edited form as: Toxicology. 2018 May 3;404-405:25–32. doi: 10.1016/j.tox.2018.05.003

Fig. 1.

Fig. 1

Characterization of a human squamous cell carcinoma cell line (A431) over-expressing human PPARβ/δ or PPARγ. (A) Representative photomicrographs of A431 cells, A431-Migr1 control cells (Migr1), A431-Migr1-hPPARβ/δ cells (hPPARβ/δ), and A431-Migr1-hPPARγ cells (hPPARγ) examined by fluorescent microscopy (upper panels) or light microscopy (lower panels). qPCR analysis for mRNA expression of (B) PPARβ/δ or (C) PPARγ in the A431 cell lines, normalized to GAPDH mRNA. (D) Western blot analysis of PPARβ/δ or PPARγ in the A431 cell lines, normalized to ACTIN expression. +positive control: cell lysate from COS-1 cells transfected with hPPARβ/δ or hPPARγ expression vector. qPCR analysis of ANGPTL4 mRNA in response to (E) the PPARβ/δ ligand GW0742 for 8 h or (F) the PPARγ ligand rosiglitazone (Rosi) for 24 h, normalized to the GAPDH mRNA. Data represents triplicate independent sample means ± S.E.M.. Values with different letters are significantly different (p ≤ 0.05).