Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jun 25;11:3671–3684. doi: 10.2147/OTT.S163535

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018 Ji et al. This work is published and licensed by Dove Medical Press Limited

The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

PMC Copyright notice

PF403-induced cell cycle arrest and apoptosis in GBM cells. (A) Exposure to various concentrations of PF403 for 48 hours; U251/TMZ cells were stained with PI for flow cytometry analysis. (B) Protein levels of cyclin D1 and CDK6 were detected via immunoblotting, following the exposure of U251/TMZ cells to PF403 for 48 hours. (C) Exposure to various concentrations of PF403 for 48 hours; T98G cells were stained with PI for flow cytometry analysis. (D) Protein levels of cyclin D1 and CDK6 were detected via immunoblotting, following exposure of T98G cells to PF403 for 48 hours. (E) Exposure to various concentrations of PF403 for 48 hours; T98G cells were stained with annexin V-FITC and PI for flow cytometry analysis. (F) Protein levels of full length PARP, Bcl-2, and cleaved caspase-3 were detected via immunoblotting in T98G cells, following exposure to PF403 for 48 hours.

Abbreviations: CDK6, cell division protein kinase 6; FITC, fluorescein isothiocyanate; GBM, glioblastoma multiforme; PARP, poly ADP ribose polymerase; PF403, 13a(S)-3-hydroxyl-6,7-dimethoxyphenanthro[9,10-b]-indolizidine; PI, propidium iodide; TMZ, temozolomide.

PF403-induced cell cycle arrest and apoptosis in GBM cells. (A) Exposure to various concentrations of PF403 for 48 hours; U251/TMZ cells were stained with PI for flow cytometry analysis. (B) Protein levels of cyclin D1 and CDK6 were detected via immunoblotting, following the exposure of U251/TMZ cells to PF403 for 48 hours. (C) Exposure to various concentrations of PF403 for 48 hours; T98G cells were stained with PI for flow cytometry analysis. (D) Protein levels of cyclin D1 and CDK6 were detected via immunoblotting, following exposure of T98G cells to PF403 for 48 hours. (E) Exposure to various concentrations of PF403 for 48 hours; T98G cells were stained with annexin V-FITC and PI for flow cytometry analysis. (F) Protein levels of full length PARP, Bcl-2, and cleaved caspase-3 were detected via immunoblotting in T98G cells, following exposure to PF403 for 48 hours.

Abbreviations: CDK6, cell division protein kinase 6; FITC, fluorescein isothiocyanate; GBM, glioblastoma multiforme; PARP, poly ADP ribose polymerase; PF403, 13a(S)-3-hydroxyl-6,7-dimethoxyphenanthro[9,10-b]-indolizidine; PI, propidium iodide; TMZ, temozolomide.