Figure 5.

CAT₃-inhibited tumor growth of TMZ-resistant GBM in vivo. (A) MRI T2-weighted image of intracranial tumors from various groups of the U251/TMZ orthotopic model. TMZ (50 mg/kg) was orally administered to mice for 5 days or CAT₃ was administered daily. (B) Tumor volumes in the U251/TMZ orthotopic model. *P < 0.05, compared to the vehicle group, ANOVA analysis. (C) Histological analysis of tumor tissues in the mouse brain of the U251/TMZ orthotopic model. H&E staining (200×), Gli1 staining (200×). (D) Tumor growth curve in T98G subcutaneous xenograft model. TMZ was orally administered (30 mg/kg or 50 mg/kg) to mice for 5 days or CAT₃ was administered daily. *P < 0.05 and ***P < 0.001, compared to the vehicle group, ANOVA analysis. (E) Tumor weight in individual mice in the T98G subcutaneous xenograft model. *P < 0.05, **P < 0.01, and ***P < 0.001, compared to the vehicle group, ANOVA analysis. (F) Tumor tissues from the T98G subcutaneous xenograft model were collected and Gli1, Smo, and MGMT levels were detected via immunoblotting. (G) Histological analysis of tumor tissues in the T98G subcutaneous xenograft model. Gli1 staining (200×).

Abbreviations: ANOVA, analysis of variance; GBM, glioblastoma multiforme; H&E, hematoxylin and eosin; MGMT, O⁶-methylguanine DNA methyltransferase; Smo, smoothened; TMZ, temozolomide.