Table 1.
Mean relative abundance of background amplification from mock community cells extracted using the RBBC method for DNA extraction (MC) in comparison to mock community DNA (DS) amplified with two primer sets targeting the V3–V4 and V4 region of the 16S rRNA gene at both 20 and 28 cycles.
| DNA extraction |
|||||
|---|---|---|---|---|---|
| DS | SEM | MC | SEM | P-value | |
| BLAST_V3–V4_20 | 0.16 | 0.01 | 0.57 | 0.03 | <0.05 |
| BLAST_V3–V4_28 | 0.96 | 0.03 | 1.47 | 0.06 | <0.05 |
| BLAST_V4_20 | 0.17 | 0.01 | 0.56 | 0.08 | <0.05 |
| BLAST_V4_28 | 0.63 | 0.03 | 1.19 | 0.04 | <0.05 |
P-values were calculated using a Mann–Whitney U-test to evaluate background amplification from each treatment group (n = 5) resulting from DNA extraction method.