Table 3.
Mean relative abundance of background amplification resulting from the number of PCR cycles during library preparation; 20 and 28 PCR cycles, constructed from DNA extracted from mock community cells extracted using the RBBC method (MC) and mock community DNA (DS) amplified with two primer sets targeting the V3–V4 and V4 region of the 16S rRNA gene.
| PCR cycle number |
|||||
|---|---|---|---|---|---|
| 20 PCR1 | SEM | 28 PCR1 | SEM | P-value | |
| BLAST_DS_V4 | 0.17 | 0.01 | 0.63 | 0.03 | <0.05 |
| BLAST_DS_V3–V4 | 0.16 | 0.01 | 0.96 | 0.03 | <0.05 |
| BLAST_MC_V4 | 0.56 | 0.08 | 1.19 | 0.04 | <0.05 |
| BLAST_MC_V3–V4 | 0.57 | 0.03 | 1.47 | 0.06 | <0.05 |
P-values were calculated using a Mann–Whitney U-test to evaluate background amplification from each treatment group (n = 5) resulting from PCR cycle number.