Figure 1.

Experimental plan of infusion and sampling. One week prior to infusion, milk was collected from all udder quarters, and somatic cell counts were determined in order to assess health status. One healthy quarter per animal was selected for infusion. On day 6, cows were transferred from the loose housing system to a tie-stall. On day 7, lipopolysaccharide or physiological saline solution was infused and sampling was performed as indicated. Follow-up sampling was performed for 1 week after infusion. Abbreviations: AMR, automatic milking rotary; VMS, Voluntary Milking System; h.p.i, hours post-infusion.