FIGURE 1.

Restrictive labeling of the fru-Gal4 line using the FLP library. (A) Schematic representation of our intersectional genetics approach using FLP and GAL4 to target fru subpopulations. (B) Expression pattern of the complete fru circuit in the CNS. (C,D) Tetanus toxin is expressed in fru neurons targeted by combining lines from the FLP library with UAS>stop>TNT and fru-GAL4. (Left Panel): Histogram depicting the fertility index distribution of the complete tetanus toxin screens containing 67 FLP lines that showed restricted GFP expression when combined with UAS>stop>mCD8::GFP and fru-GAL4. (Right Panel): Sample expression profile in each fertility category. Although the restricted fru expression profiles should be present in the full circuit image in (B), it may not be apparent due to variation in intensity and overlapping signals. Tissues were stained with anti-mCD8 (green) and anti-nC82 (magenta). Scale Bar = 50 μm.